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Coding

Part:BBa_K322118

Designed by: Meng Lu   Group: iGEM10_Edinburgh   (2010-10-27)


CcaS upstream + PhoR downstream

CcaS is a green light receptor, which has very similar downstream with the upstream of PhoR. The upstream region of CcaS senses green light, then phosphorylating the fused downstream region of PhoR. PhoR interacts with PhoB, which regulates PhoA promoter.

In the presence green light, genes regulated by PhoB should be repressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1235
    Illegal EcoRI site found at 1528
    Illegal SpeI site found at 1330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1235
    Illegal EcoRI site found at 1528
    Illegal NheI site found at 606
    Illegal SpeI site found at 1330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1235
    Illegal EcoRI site found at 1528
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1235
    Illegal EcoRI site found at 1528
    Illegal SpeI site found at 1330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1235
    Illegal EcoRI site found at 1528
    Illegal SpeI site found at 1330
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


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